Evaluation of in vitro Antibacterial and Antioxidant Activity of Aqueous Extracts of Olax psittacorum
By: Majumder, R
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Contributor(s): Dhara, Moonmun | Adhikari, Lopamudra.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2019Edition: Vol. 81(1), Jan-Feb.Description: 99-109p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Present study aimed to evaluate the phytoconstituents of the aqueous extracts of stem and fruits of Olax psittacorum as well as the free radical scavenging and the antibacterial activity of the extracts. Free radical scavenging activities were evaluated through 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)di-ammonium salt radical scavenging assay, phosphomolybdate radical scavenging assay, hydrogen peroxide assay and reducing power assay using ascorbic acid as the standard. Well diffusion method was adopted for antibacterial activity evaluation of the aqueous extract of fruits and stem against Staphylococcus aureus, Bacillus stereothermophillus, Pseudomonas aeruginosa, Vibrio cholera, Escherichia coli and Acinetobacter baumanii. Phytochemical screening showed presence of tannins, saponins, steroids and terpenoids in the aqueous extracts of both stem and fruits, while glycosides, flavonoids, carbohydrates and reducing sugars were found in the aqueous extract of fruit only. Total phenolic, tannin and saponin contents were found to be higher in the aqueous extract of fruits. Total flavonoid content of aqueous extract of fruit was found to be 279.33 mg quercetin equivalent per gram of dry extract. Variations in phytoconstituents and in vitro experimental data obtained through antioxidant as well as antimicrobial assay methods indicated the existence of significant difference (p<0.05) between the antioxidant potency and zones of inhibition of Staphylococcus aureus, Vibrio cholera, Acinetobacter baumanii, which aqueous extract of stem failed to show at a dose of 100 mg/ml clearly demonstrated the superiority of the aqueous extract of fruits
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Present study aimed to evaluate the phytoconstituents of the aqueous extracts of stem and fruits of Olax psittacorum as well as the free radical scavenging and the antibacterial activity of the extracts. Free radical scavenging activities were evaluated through 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)di-ammonium salt radical scavenging assay, phosphomolybdate radical scavenging assay, hydrogen peroxide assay and reducing power assay using ascorbic acid as the standard. Well diffusion method was adopted for antibacterial activity evaluation of the aqueous extract of fruits and stem against Staphylococcus aureus, Bacillus stereothermophillus, Pseudomonas aeruginosa, Vibrio cholera, Escherichia coli and Acinetobacter baumanii. Phytochemical screening showed presence of tannins, saponins, steroids and terpenoids in the aqueous extracts of both stem and fruits, while glycosides, flavonoids, carbohydrates and reducing sugars were found in the aqueous extract of fruit only. Total phenolic, tannin and saponin contents were found to be higher in the aqueous extract of fruits. Total flavonoid content of aqueous extract of fruit was found to be 279.33 mg quercetin equivalent per gram of dry extract. Variations in phytoconstituents and in vitro experimental data obtained through antioxidant as well as antimicrobial assay methods indicated the existence of significant difference (p<0.05) between the antioxidant potency and zones of inhibition of Staphylococcus aureus, Vibrio cholera, Acinetobacter baumanii, which aqueous extract of stem failed to show at a dose of 100 mg/ml clearly demonstrated the superiority of the aqueous extract of fruits
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